Mouse Monoclonal Antibody to DiMethyl-Histone H3 (Lys27)

Description
H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.

Specification
Aliases	H3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Entrez GeneID	8350
Swissprot	P68431
clone	5H6
WB Predicted band size	Calculated MW: 15 kDa; Observed MW: 15 kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthetic Peptide of Histone H3 (Di Methyl Lys27)
Formulation	Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application
WB	1/500-1/1000
IP	1/10-1/20
IF	1/50-1/200