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Mouse Monoclonal Antibody to ADORA2A
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货号:
31299 -
别名:
A2aR; RDC8; ADORA2 -
应用:
WB,IF,FCM -
反应种属:
Human,Mouse,Rat,Monkey -
抗体类型:
Primary antibody -
Swissprot:
P29274 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180

Mouse Monoclonal Antibody to ADORA2A
Description |
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This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding. |
References |
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1.Mol Neurobiol. 2015 Aug;52(1):664-78. 2.J Psychiatr Res. 2014 Apr;51:49-59. |
Specification |
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Aliases | A2aR; RDC8; ADORA2 |
Entrez GeneID | 135 |
Swissprot | P29274 |
clone | 6A6G11 |
WB Predicted band size | 44.7kDa |
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat,Monkey |
Immunogen | Purified recombinant fragment of human ADORA2A (AA: 274-412) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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WB | 1/500-1/1000 |
IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using ADORA2A mouse mAb against CHO3D10(1),COS7(2),F9(3),L1210(4),C6(5),C2C12(6),NIH/3T3(7),Raw264.7(8) cell lysate.
- Immunofluorescence analysis of Hela cells using ADORA2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using ADORA2A mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31299

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