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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ALDOA mouse mAb against MCF-7 (1), Hela (2), and NIH/3T3 (3) cell lysate.
Flow cytometric analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using ALDOA mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ALDOA mouse mAb with DAB staining.

Mouse Monoclonal Antibody to ALDOA

  • 货号:

    31287
  • 别名:

    ALDA; GSD12; HEL-S-87p
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human,Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P04075
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to ALDOA

Description

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.

References

1.Cancer Lett. 2016 Apr 28;374(1):127-35.
2.Oncol Rep. 2014 Nov;32(5):2031-7.

Specification

Aliases ALDA; GSD12; HEL-S-87p
Entrez GeneID 226
Swissprot P04075
clone 1C5B2
WB Predicted band size 39.4kDa
Host/Isotype Mouse IgG2a
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen Purified recombinant fragment of human ALDOA (AA: 9-145) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000