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        Mouse Monoclonal Antibody to ATG3
- 
										
货号:
31200 - 
										
别名:
APG3; APG3L; PC3-96; APG3-LIKE - 
										
应用:
WB,IHC,IF,FCM - 
										
反应种属:
Human - 
										
抗体类型:
Primary antibody - 
										
Swissprot:
Q9NT62 - 
										
规格:
 - 
										
数量:
-+ - 
										
										
说明书:
目录价¥2180
 
						        
						        
						        
						        
						    Mouse Monoclonal Antibody to ATG3
Description | 
									
|---|
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. | 
									
References | 
									
|---|
| 1.Mol Biol Rep. 2014;41(4):2093-9.  2.Apoptosis. 2012 Aug;17(8):810-20.  | 
									
Specification | 
									|
|---|---|
| Aliases | APG3; APG3L; PC3-96; APG3-LIKE | 
| Entrez GeneID | 64422 | 
| Swissprot | Q9NT62 | 
| clone | 2C10A12 | 
| WB Predicted band size | 35.9kDa | 
| Host/Isotype | Mouse IgG1 | 
| Antibody Type | Primary antibody | 
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. | 
| Species Reactivity | Human | 
| Immunogen | Purified recombinant fragment of human ATG3 (AA: 1-100) expressed in E. Coli. | 
| Formulation | Purified antibody in PBS with 0.05% sodium azide | 
Application | 
									|
|---|---|
| WB | 1/500 - 1/2000 | 
| IHC | 1/200 - 1/1000 | 
| IF/ICC | 1/200 - 1/1000 | 
| FCM | 1/200 - 1/400 | 
| ELISA | 1/10000 | 
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
 - Western blot analysis using ATG3 mouse mAb against K562 (1), Hela (2), and THP-1 (3) cell lysate.
 - Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
 - Flow cytometric analysis of Jurkat cells using ATG3 mouse mAb (green) and negative control (red).
 - Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using ATG3 mouse mAb with DAB staining.
 - Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATG3 mouse mAb with DAB staining.
 
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31200
						
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