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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using ATG3 mouse mAb against K562 (1), Hela (2), and THP-1 (3) cell lysate.

Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using ATG3 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using ATG3 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATG3 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to ATG3

货号：
31200
别名：
APG3; APG3L; PC3-96; APG3-LIKE
产品上架时间：
2024-01-01
应用：
WB,IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9NT62
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to ATG3

Description
This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.

References
1.Mol Biol Rep. 2014;41(4):2093-9. 2.Apoptosis. 2012 Aug;17(8):810-20.

Specification
Aliases	APG3; APG3L; PC3-96; APG3-LIKE
Entrez GeneID	64422
Swissprot	Q9NT62
clone	2C10A12
WB Predicted band size	35.9kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human ATG3 (AA: 1-100) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide