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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ATF4 mouse mAb against K562 (1), A431 (2), Hela (3), HEK293 (4), and Ramos (5) cell lysate.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ATF4 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using ATF4 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to ATF4

  • 货号:

    31162
  • 别名:

    CREB2; TXREB; CREB-2; TAXREB67
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P18848
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to ATF4

Description

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

References

1.Cell. 2014 Aug 28;158(5):1159-72.
2.Tumour Biol. 2014 Jan;35(1):765-71.

Specification

Aliases CREB2; TXREB; CREB-2; TAXREB67
Entrez GeneID 468
Swissprot P18848
clone 2A6F12
WB Predicted band size 38.6kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human ATF4 (AA: 212-351) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ELISA 1/10000