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Mouse Monoclonal Antibody to UHRF1
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货号:
31125 -
别名:
Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95 -
产品上架时间:
2024-01-01 -
应用:
WB,IHC,IF,FCM -
反应种属:
Human -
抗体类型:
Primary antibody -
Swissprot:
Q96T88 -
规格:
-
数量:
-+ -
说明书:
目录价¥2180
Mouse Monoclonal Antibody to UHRF1
Description |
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This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. |
References |
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1.Biomarkers. 2015;20(3):183-8. 2.Med Oncol. 2013 Dec;30(4):613. |
Specification |
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Aliases | Np95; hNP95; ICBP90; RNF106; TDRD22; hUHRF1; huNp95 |
Entrez GeneID | 29128 |
Swissprot | Q96T88 |
clone | 1A1B1 |
WB Predicted band size | 89.8kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human UHRF1 (AA: 616-755) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using UHRF1 mouse mAb against MCF-7 (1) and Hela (2) cell lysate.
- Immunofluorescence analysis of Hela cells using UHRF1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Immunofluorescence analysis of Hela cells using UHRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of MCF-7 cells using UHRF1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using UHRF1 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using UHRF1 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31125
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