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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.
Flow cytometric analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to IDH1

  • 货号:

    31106
  • 别名:

    IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,FCM
  • 反应种属:

    Human,Mouse,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    O75874
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to IDH1

Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

References

1.Cancer Cell. 2015 Dec 14;28(6):773-84.
2.Int J Cancer. 2015 Sep 1;137(5):1058-65.

Specification

Aliases IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26
Entrez GeneID 3417
Swissprot O75874
clone 4A4A8
WB Predicted band size 46.7kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Monkey
Immunogen Purified recombinant fragment of human IDH1 (AA: 156-298) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000