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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.
Immunofluorescence analysis of Hela cells using MIB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to MIB1
-
货号：
31100 -
别名：
MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2 -
应用：
WB,IF,FCM -
反应种属：
Human,Monkey -
抗体类型：
Primary antibody -
Swissprot：
Q86YT6 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to MIB1
Description |
|---|
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). |
References |
|---|
| 1.J Cell Sci. 2015 May 1;128(9):1674-82. 2.Cell Res. 2012 Mar;22(3):603-6. |
Specification |
|
|---|---|
| Aliases | MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2 |
| Entrez GeneID | 57534 |
| Swissprot | Q86YT6 |
| clone | 6A9C9 |
| WB Predicted band size | 110kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human MIB1 (AA: 6-221) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using MIB1 mouse mAb against Hela (1) and COS7 (2) cell lysate.
- Immunofluorescence analysis of Hela cells using MIB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using MIB1 mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31100
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