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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using DDX5 mouse mAb against HT-29 (1), Hela (2), NIH/3T3 (3), COS7 (4), SW620 (5), Jurkat (6), A431 (7), and MCF-7 (8) cell lysate.
Flow cytometric analysis of Hela cells using DDX5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using DDX5 mouse mAb with DAB staining.
HOT

Mouse Monoclonal Antibody to DDX5

  • 货号:

    30987
  • 别名:

    p68; HLR1; G17P1; HUMP68
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human,Mouse,Monkey
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P17844
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to DDX5

Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified.

References

1.Cancer Discov. 2012 Sep;2(9):812-25.
2.Nucleic Acids Res. 2012 Apr;40(7):3159-71.

Specification

Aliases p68; HLR1; G17P1; HUMP68
Entrez GeneID 1655
Swissprot P17844
clone 4F1C12
WB Predicted band size 69.1kDa
Host/Isotype Mouse IgG2a
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Monkey
Immunogen Purified recombinant fragment of human DDX5 (AA: 475-614) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000