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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).
Rat Monoclonal Antibody to phospho-NLRC4(Ser-533)
-
货号：
30944 -
别名：
CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik -
应用：
IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q3UP24 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rat Monoclonal Antibody to phospho-NLRC4(Ser-533)
Description |
|---|
NLRC4 is a cytosolic NOD (nucleotide binding and oligomerization domain)-like receptor (NLR) that can trigger inflammasome formation in response to bacterial flagellin, an immunodominant antigen in the intestine. |
References |
|---|
| 1.Mucosal Immunol. 2014 Jul;7(4):775-85. 2.Mucosal Immunol. 2012 May;5(3):288-98. |
Specification |
|
|---|---|
| Aliases | CLAN; IPAF; CLAN1; CLANA; CLANB; CLANC; CLAND; Card12; 9530011P19Rik |
| Entrez GeneID | 268973 |
| Swissprot | Q3UP24 |
| clone | 9A6A1 |
| WB Predicted band size | 116.7kDa |
| Host/Isotype | Rat IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of mouse phospho-NLRC4(Ser-533) (AA: 525-538) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Immunofluorescence analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).
- Flow cytometric analysis of HepG2 cells using phospho-NLRC4(Ser-533) rat mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30944
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