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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using DDX20 mouse mAb against Jurkat (1), Hela (2), NTERA-2 (3), HL7702 (4), K562 (5), and C6 (6) cell lysate.
Immunofluorescence analysis of HeLa cells using DDX20 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HeLa cells using DDX20 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to DDX20

  • 货号:

    30899
  • 别名:

    DP103; GEMIN3
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,FCM
  • 反应种属:

    Human,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9UHI6
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to DDX20

Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.

References

1.J Clin Invest. 2014 Sep;124(9):3807-24.
2.J Biol Chem. 1999 Jul 2;274(27):19136-44.

Specification

Aliases DP103; GEMIN3
Entrez GeneID 11218
Swissprot Q9UHI6
clone 1C9F6
WB Predicted band size 92.2kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Rat
Immunogen Purified recombinant fragment of human DDX20 (AA: 725-824) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000