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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to NOS1

  • 货号:

    30862
  • 别名:

    NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
  • 产品上架时间:

    2024-01-01
  • 应用:

    IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P29475
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to NOS1

Description

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.

References

1.J Neurochem. 2013 Aug;126(3):318-30.
2.J Leukoc Biol. 2012 Jun;91(6):947-56.

Specification

Aliases NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS
Entrez GeneID 4842
Swissprot P29475
clone 2E11G6
WB Predicted band size 161kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human NOS1 (AA: 17-153) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

IF 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000