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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western blot analysis using WTAP mouse mAb against MCF-7 (1), Hela (2), K562 (3), Hek293 (4), A549 (5), HepG2 (6), Jurkat (7), and Cos7 (8) cell lysate.

Western blot analysis using WTAP mouse mAb against PC-12(1)NIH/3T3(2) NRK(3) C2C12 (4) C6(5) L1210(6) F9(7) COS7(8) CHO3D10(9) cell lysate.

Immunofluorescence analysis of Hela cells using WTAP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of A549 cells using WTAP mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using WTAP mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using WTAP mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded Mouse testicles(A) Mouse cerebellum(B) using WTAP mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded Rat brain(A) Rat testicles(B) using WTAP mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded Rabbit testicles(A) Rabbit cerebellum(b) using WTAP mouse mAb with DAB staining.

Mouse Monoclonal Antibody to WTAP

货号：
30788
别名：
PNAS-132;hFL(2)D;KIAA0105
产品上架时间：
2024-03-20
应用：
WB,IHC,IF,FCM
反应种属：
Human,Mouse,Rat,Rabbit,Monkey
抗体类型：
Primary antibody
Swissprot：
Q15007
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥2180

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Mouse Monoclonal Antibody to WTAP

Description
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.

Description

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms.

References
1.J Gastroenterol. 2013 Nov;48(11):1271-82. 2.Cancer Sci. 2012 Dec;103(12):2102-9.

Specification
Aliases	PNAS-132;hFL(2)D;KIAA0105
Entrez GeneID	9589
Swissprot	Q15007
clone	6B6B6
WB Predicted band size	44.2kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Rabbit,Monkey
Immunogen	Purified recombinant fragment of human WTAP (AA: 91-201) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide