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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.

Rat Monoclonal Antibody to PKHD1

  • 货号:

    30740
  • 别名:

    FPC; Tigm1; AI118496; AI182499
  • 产品上架时间:

    2024-01-01
  • 应用:

    IHC,ICC,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    E9PZ36
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rat Monoclonal Antibody to PKHD1

Description

Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, the lack of specific antibodies to the mouse protein impairs the study of splicing, post-translational processing, shedding, and temporal and spatial expression of endogenous fibrocystin at the cellular and subcellular level.

References

J Am Soc Nephrol. 2011 Dec;22(12):2266-77.
Am J Pathol. 2008 Feb;172(2):417-29.

Specification

Aliases FPC; Tigm1; AI118496; AI182499
Entrez GeneID 241035
Swissprot E9PZ36
clone 8G12A1
WB Predicted band size 445kDa
Host/Isotype Rat IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of mouse PKHD1 (AA: 3878-4060) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000