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Western blot analysis of lysate from MDA-MB-231 cell line, using EDG4 Antibody (N18)(Cat. #P32780). P32780 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
The anti-EDG4 N-term Antibody (Cat.#P32780) is used in Western blot to detect EDG4 in HL60 lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Rabbit Polyclonal Antibody to EDG4 (N-term)

  • 货号:

    P32780
  • 别名:

    Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2, Lysophosphatidic acid receptor Edg-4, LPAR2, EDG4, LPA2
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q9HBW0
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to EDG4 (N-term)

Description

EDG4 is a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins.

Specification

Aliases Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2, Lysophosphatidic acid receptor Edg-4, LPAR2, EDG4, LPA2
Entrez GeneID 9170
Swissprot Q9HBW0
WB Predicted band size 39.1kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This EDG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of human EDG4.
Formulation Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) .

Application

WB 1/1000
IHC 1/100-1/500