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Western blot analysis of lysate from HUVEC cell line, using (DANRE) gapdh Antibody (C-term)(Cat. #P32698). P32698 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Rabbit Polyclonal Antibody to (DANRE) gapdh

  • 货号:

    P32698
  • 别名:

    Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1}
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q5XJ10
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to (DANRE) gapdh

Description

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).

Specification

Aliases Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, gapdh {ECO:0000312|ZFIN:ZDB-GENE-030115-1}
Entrez GeneID 317743
Swissprot Q5XJ10
WB Predicted band size 35.8kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This (DANRE) gapdh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of DANRE gapdh.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P32698