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Western blot analysis of lysates from A431, Hela, HepG2 cell line , human liver and mouse liver tissue lysate(from left to right), using ETFA Antibody (Center)(Cat. #P32649). P32649 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Fluorescent image of Hela cells stained with ETFA Antibody (Center)(Cat#P32649). P32649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Rabbit Polyclonal Antibody to ETFA
-
货号：
P32649 -
别名：
Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA -
应用：
WB,IF -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P13804 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to ETFA
Description |
|---|
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). |
Specification |
|
|---|---|
| Aliases | Electron transfer flavoprotein subunit alpha, mitochondrial, Alpha-ETF, ETFA |
| Entrez GeneID | 2108 |
| Swissprot | P13804 |
| WB Predicted band size | 35.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ETFA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 192-226 amino acids from the Central region of human ETFA. |
Application |
|
|---|---|
| WB | 1/1000 |
| IF | 1/25 |
Product Image
- Western blot analysis of lysates from A431, Hela, HepG2 cell line , human liver and mouse liver tissue lysate(from left to right), using ETFA Antibody (Center)(Cat. #P32649). P32649 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
- Fluorescent image of Hela cells stained with ETFA Antibody (Center)(Cat#P32649). P32649 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32649
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