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Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term)(Cat. #P32638). P32638 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green, Cat#P32638) compared to an isotype control of rabbit IgG(blue). P32638 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Mouse Mapk3 Antibody (C-term) (P32638)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Rabbit Polyclonal Antibody to Mouse Mapk3

  • 货号:

    P32638
  • 别名:

    Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1, Mapk3, Erk1, Prkm3
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human, Mouse, Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q63844
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to Mouse Mapk3

Description

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.

Specification

Aliases Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, MNK1, Microtubule-associated protein 2 kinase, p44-ERK1, Mapk3, Erk1, Prkm3
Entrez GeneID 26417
Swissprot Q63844
WB Predicted band size 43.1kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3.
Formulation Purified antibody in PBS with 0.05% sodium azide.

Application

WB 1/1000
IHC 1/100-1/500
FCM 1/25