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Western blot analysis of lysates from A549, A431, SK-BR-3 cell line (from left to right), using CAPN2 Antibody(Cat. #P32636). P32636 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Flow cytometric analysis of U-87 MG cells using CAPN2 Antibody(green, Cat#P32636) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H.stomach section using CAPN2 Antibody(Cat#P32636). P32636 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Mouse Monoclonal Antibody to CAPN2

  • 货号:

    P32636
  • 别名:

    Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human, Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P17655
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to CAPN2

Description

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Specification

Aliases Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2
Entrez GeneID 824
Swissprot P17655
WB Predicted band size 80.0kDa
Host/Isotype Mouse IgG2b
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2.

Application

WB 1/1000
IHC 1/100-1/500
FCM 1/100