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Western blot analysis of lysate from HT-1080 cell line, using AGAP8 Antibody (C-term)(Cat. # P32627). P32627 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
Fluorescent image of HeLa cells stained with AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green, Cat#P32627) compared to an isotype control of rabbit IgG(blue). P32627 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Rabbit Polyclonal Antibody to AGAP8
-
货号：
P32627 -
别名：
Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 8, AGAP-8, Centaurin-gamma-like family member 5, AGAP8, CTGLF5 -
应用：
WB,IHC,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q5SRD3 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to AGAP8
Description |
|---|
Putative GTPase-activating protein (Potential). |
Specification |
|
|---|---|
| Aliases | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 8, AGAP-8, Centaurin-gamma-like family member 5, AGAP8, CTGLF5 |
| Swissprot | Q5SRD3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AGAP8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 498-531 amino acids from the C-terminal region of human AGAP8. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
- Western blot analysis of lysate from HT-1080 cell line, using AGAP8 Antibody (C-term)(Cat. # P32627). P32627 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Fluorescent image of HeLa cells stained with AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
- Flow cytometric analysis of MCF-7 cells using AGAP8 Antibody (C-term)(green, Cat#P32627) compared to an isotype control of rabbit IgG(blue). P32627 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
- Immunohistochemical analysis of paraffin-embedded H. liver section using AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
- Immunohistochemical analysis of paraffin-embedded M. liver section using AGAP8 Antibody (C-term)(Cat#P32627). P32627 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P32627
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