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Western blot analysis of lysates from Hela, K562 cell line (from left to right), using EEF1B2 Antibody (Center)(Cat. #P32608). P32608 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Fluorescent image of Hela cells stained with EEF1B2 Antibody (Center)(Cat#P32608). P32608 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Flow cytometric analysis of Hela cells using EEF1B2 Antibody (Center)(green, Cat#P32608) compared to an isotype control of rabbit IgG(blue). P32608 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded H. brain section using EEF1B2 Antibody (Center)(Cat#P32608). P32608 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Rabbit Polyclonal Antibody to EEF1B2

  • 货号:

    P32608
  • 别名:

    Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,IF,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P24534
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to EEF1B2

Description

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

Specification

Aliases Elongation factor 1-beta, EF-1-beta, EEF1B2, EEF1B, EF1B
Entrez GeneID 1933
Swissprot P24534
WB Predicted band size 24.8kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This EEF1B2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 54-86 amino acids from the Central region of human EEF1B2.

Application

WB 1/1000
IHC 1/100-1/500
IF 1/25
FCM 1/25