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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using BAD mouse mAb against MCF-7 (1), HEK293 (2) cell lysate.
Flow cytometric analysis of MCF-7 cells using BAD mouse mAb (green) and negative control (red).
Mouse Monoclonal Antibody to BAD
-
货号：
30637 -
别名：
BBC2; BCL2L8 -
应用：
WB,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q92934 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to BAD
Description |
|---|
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. |
References |
|---|
| 1. Clin Cancer Res. 2011 Oct 1;17(19):6356-66. 2. Mol Cancer Res. 2010 Aug;8(8):1116-25. |
Specification |
|
|---|---|
| Aliases | BBC2; BCL2L8 |
| Entrez GeneID | 572 |
| Swissprot | Q92934 |
| clone | 1G5B3 |
| WB Predicted band size | 18.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BAD (AA: FULL(1-168)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using BAD mouse mAb against MCF-7 (1), HEK293 (2) cell lysate.
- Flow cytometric analysis of MCF-7 cells using BAD mouse mAb (green) and negative control (red).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30637
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