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RFX1 Antibody (C-term) (Cat. #P31698) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow).
Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat#P31698).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).RFX1 immunoreactivity is localized to Nucleus significantly.
Rabbit Polyclonal Antibody to RFX1
-
货号：
P31698 -
别名：
MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1, RFX1 -
应用：
WB,FCM -
反应种属：
Human, Mouse -
抗体类型：
Primary antibody -
Swissprot：
P22670 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to RFX1
Description |
|---|
This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression. Also, it can bind to an inverted repeat that is required for expression of hepatitis B virus genes. |
Specification |
|
|---|---|
| Aliases | MHC class II regulatory factor RFX1, Enhancer factor C, EF-C, Regulatory factor X 1, RFX, Transcription factor RFX1, RFX1 |
| Entrez GeneID | 5989 |
| Swissprot | P22670 |
| WB Predicted band size | 104.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RFX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 896-925 amino acids from the C-terminal region of human RFX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
Product Image
- RFX1 Antibody (C-term) (Cat. #P31698) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the RFX1 antibody detected the RFX1 protein (arrow).
- Fluorescent image of U251 cell stained with RFX1 Antibody (C-term)(Cat#P31698).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RFX1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).RFX1 immunoreactivity is localized to Nucleus significantly.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P31698
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