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Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab (Cat. #P31563) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P31563 phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.
Rabbit Polyclonal Antibody to Phospho-GATA6(Y271)
-
货号：
P31563 -
别名：
GATA6; Transcription factor GATA-6; GATA-binding factor 6 -
应用：
WB -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q92908 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Phospho-GATA6(Y271)
Description |
|---|
Thought to be important for regulating terminal differentiation and/or proliferation. |
Specification |
|
|---|---|
| Aliases | GATA6; Transcription factor GATA-6; GATA-binding factor 6 |
| Entrez GeneID | 2627 |
| Swissprot | Q92908 |
| WB Predicted band size | 60.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Phospho-GATA6-Y271 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding Y271 of human GATA6, isoform 2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | DB: 1/500 |
Product Image
- Dot blot analysis of Phospho-GATA6-pY271 Phospho-specific Pab (Cat. #P31563) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
- Fluorescent confocal image of HepG2 cells stained with phospho-GATA6-Y271 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P31563 phospho-GATA6-Y271 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho-GATA6 immunosignal mainly to the nucleus.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P31563
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