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Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (Cat. #P31476) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P31476 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
Rabbit Polyclonal Antibody to Phospho-LIN28(S134)
-
货号：
P31476 -
别名：
Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1 -
应用：
WB -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9H9Z2 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Polyclonal Antibody to Phospho-LIN28(S134)
Description |
|---|
LIN28 is probable transcription factor. It plays a critical role in the control of immune response. |
Specification |
|
|---|---|
| Aliases | Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1 |
| Entrez GeneID | 79727 |
| Swissprot | Q9H9Z2 |
| WB Predicted band size | 22.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LIN28 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S134 of human LIN28. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | DB: 1/500 |
Product Image
- Dot blot analysis of anti-Phospho-LIN28-S134 Phospho-specific Pab (Cat. #P31476) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Fluorescent confocal image of SY5Y cells stained with phospho- LIN28- S134 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with P31476 phospho- LIN28- S134 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the phospho- LIN28 immunosignal mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000131914).
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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