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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using EGFR mouse mAb against A431 (1) AND Hela (2) cell lysate.
Western blot analysis using EGFR mouse mAb against C2C12(1),81505(2) cell lysate.
Flow cytometric analysis of A431 cells using EGFR mouse mAb (green) and negative control (red).

Mouse Monoclonal Antibody to EGFR

  • 货号:

    30551
  • 别名:

    ERBB; HER1; mENA; ERBB1; PIG61
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,FCM
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P00533
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to EGFR

Description

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

References

1. J Immunol. 2012 Dec 1;189(11):5230-9.
2. J Biol Chem. 2012 Oct 12;287(42):35201-11.

Specification

Aliases ERBB; HER1; mENA; ERBB1; PIG61
Entrez GeneID 1956
Swissprot P00533
clone 5E10D3
WB Predicted band size 175kDa
Host/Isotype Mouse IgG2b
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Purified recombinant fragment of human EGFR (AA: 693-893) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
FCM 1/200 - 1/400
ELISA 1/10000