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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), Hela (3) and HepG2 (4) cell lysate.
Flow cytometric analysis of Hela cells using CK5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CK5 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human stomach tissues using CK5 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to CK5

  • 货号:

    30496
  • 别名:

    K5; KRT5; DDD; EBS2; KRT5A
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC,FCM
  • 反应种属:

    Human
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P13647
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to CK5

Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

References

1.Am J Surg Pathol. 2009 Nov;33(11):1615-23.
2.J Dermatol. 2009 Aug;36(8):447-52.

Specification

Aliases K5; KRT5; DDD; EBS2; KRT5A
Entrez GeneID 3852
Swissprot P13647
clone 2C2B4
WB Predicted band size 62.3kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human CK5 (AA: 316-590) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IHC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000