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PAX6 Antibody (Center) (Cat. #P30426) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
Confocal immunofluorescent analysis of PAX6 Antibody (Center)(Cat#P30426) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Polyclonal Antibody to PAX6
-
货号：
P30426 -
别名：
Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2 -
应用：
WB,IHC-P,IF,FCM -
反应种属：
Human, Mouse, Rat -
抗体类型：
Primary antibody -
Swissprot：
P26367 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to PAX6
Description |
|---|
PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. |
Specification |
|
|---|---|
| Aliases | Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2 |
| Entrez GeneID | 5080 |
| Swissprot | P26367 |
| WB Predicted band size | 46.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF/ICC | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
- PAX6 Antibody (Center) (Cat. #P30426) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
- Confocal immunofluorescent analysis of PAX6 Antibody (Center)(Cat#P30426) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
- Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P30426
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