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Western blot analysis of Src in HT-29, Hela and MDA lysates using Src (Ab529) antibody.
Western blot analysis of Src in Hela, C6, 3T3 and PC-12 lysates using Src (Ab529) antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using Src (Ab529) antibody(left) or the same antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Rabbit Polyclonal Antibody to Src

  • 货号:

    P23545
  • 别名:

    SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
  • 产品上架时间:

    2024-05-06
  • 应用:

    WB,IHC
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P12931
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1980

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Rabbit Polyclonal Antibody to Src

Description

Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Specification

Aliases SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
Entrez GeneID 6714
Swissprot P12931
WB Predicted band size Calculated MW: 60 kDa; Observed MW: 60 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen Peptide sequence around aa. 527~531 (P-Q-Y-Q-P) derived from Human Src.
Formulation Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IHC 1/50-1/100