GST Tag[20033]
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Western blot analysis using GST mouse mAb against various fusion protein with GST tag.

Mouse Monoclonal Antibody to GST Tag

  • 货号:

    20033
  • 别名:

  • 产品上架时间:

    2024-02-01
  • 应用:

    WB,IP,IF
  • 反应种属:

    Tag
  • 抗体类型:

    Tag Antibody
  • Swissprot:

  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1320

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Mouse Monoclonal Antibody to GST Tag

Description

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.

References

1. 1993. Biotechnology (N Y) 11: 933-6.
2. 1988. Gene 67: 31-40.

Specification

clone 6G9C6
Host/Isotype Mouse IgG1
Antibody Type Tag Antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Tag
Immunogen Purified fusion protein with GST protein tag expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide

Application

WB 1/500 - 1/2000
IP 1/20
IHC 1/100 - 1/1000
IF 1/100 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#20033