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Western blot analysis of Phospho-Nucleolin (T76) expression in (1) 293T cell lysate; (2) 293T cell treated with Lambda Phosphatase lysate.
Rabbit Monoclonal to Phospho-Nucleolin(T76)
-
货号：
P19081 -
别名：
MS1116; NCL; Nucl; Nucleolin; Protein C23;;p-NCL (T76) -
应用：
WB;IHC -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P19338 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to Phospho-Nucleolin(T76)
Description |
|---|
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
Specification |
|
|---|---|
| Aliases | MS1116; NCL; Nucl; Nucleolin; Protein C23;;p-NCL (T76) |
| Swissprot | P19338 |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NCL around the phosphorylation site of T76 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/200 |
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P19081
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