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Western blot analysis of Histone H3 (di methyl K4) expression in HeLa cell lysate.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
HOT

Rabbit Monoclonal to Histone H3(dimethylK4)

  • 货号:

    P18658
  • 别名:

    Histone H3.1, Histone H3, HIST1H3A;;DiMethyl-Histone H3 (K5)
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IHC/IHF,IF,IP/ChIP
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P68431
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal to Histone H3(dimethylK4)

Description

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Specification

Aliases Histone H3.1, Histone H3, HIST1H3A;;DiMethyl-Histone H3 (K5)
Swissprot P68431
WB Predicted band size 15 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthesized peptide derived from human Histone H3.1 around the methylation site of K5
Formulation Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

Application

WB 1/1000-1/2000
IP ChIP:1/20-1/50
IHC IHC:1/100-1/200;IHF:1/50-1/200
IF 1/50-1/200

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P18658