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Western blot analysis of p150 CAF1 expression in K562 cell lysate.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Rabbit Monoclonal to p150 CAF1
-
货号：
P18408 -
别名：
CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150;;CAF 1 subunit A -
应用：
WB,IHC/IHF,IF,IP,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q13111 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to p150 CAF1
Description |
|---|
Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. |
Specification |
|
|---|---|
| Aliases | CAF; CAF1; CAF1P150; CHAF1A; DCAF1; hp15; P150;;CAF 1 subunit A |
| Swissprot | Q13111 |
| WB Predicted band size | Calculated MW: 107 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human CAF 1 subunit A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF/ICC | 1/50-1/200 |
| FCM | 1/20-1/100 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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