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Western blot analysis of HIF1AN expression in Jurkat cell lysate.
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature.
Rabbit Monoclonal to HIF1AN
-
货号：
P18317 -
别名：
FIH 1; FIH1; HIF1AN;;HIF 1 alpha inhibitor -
应用：
WB,IHC/IHF,IF,FCM -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9NWT6 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to HIF1AN
Description |
|---|
Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. |
Specification |
|
|---|---|
| Aliases | FIH 1; FIH1; HIF1AN;;HIF 1 alpha inhibitor |
| Swissprot | Q9NWT6 |
| WB Predicted band size | 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human HIF 1 alpha inhibitor |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF | 1/50-1/200 |
| FCM | 1/20-1/100 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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