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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of NFE2 expression in K562 cell lysate.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
Immunofluorescent analysis using the Antibody at 1:50 dilution.
HOT
Rabbit Monoclonal to NFE2
-
货号：
P17973 -
别名：
nfe2 ; Nuclear factor; p45 NFE2;;NFE2 -
应用：
WB,IHC/IHF,IF,IP -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q16621 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to NFE2
Description |
|---|
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron. |
Specification |
|
|---|---|
| Aliases | nfe2 ; Nuclear factor; p45 NFE2;;NFE2 |
| Swissprot | Q16621 |
| WB Predicted band size | Calculated MW: 41 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human NFE2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF | 1/50-1/200 |
Product Image
- All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
- All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
- Western blot analysis of NFE2 expression in K562 cell lysate.
- Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:1K dilution)
- Immunofluorescent analysis using the Antibody at 1:50 dilution.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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