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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of UCP1+UCP3 expression in mouse skeletal muscle cell lysate.
HOT

Rabbit Monoclonal to UCP1+UCP3

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Rabbit Monoclonal to UCP1+UCP3

Description

Mitochondrial transporter that functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP.

Specification

Aliases UCP1; UCP3;;UCP1/3
Swissprot P12242/P56501(mouse)(human:P25874/P55916)
WB Predicted band size Calculated MW: 33,34 kDa ; Observed MW: 34 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Mouse,Rat
Immunogen A synthesized peptide derived from mouse UCP1/3
Formulation Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

Application

WB 1/1000-1/2000
IP 1/20-1/50

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P17856