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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Phospho-MLKL (S358) expression in (1) HT-29 cell lysate; (2) HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD.
HOT
Rabbit Monoclonal to Phospho-MLKL(S358)
-
货号：
P17797 -
别名：
hMLKL; Mlkl;;p-MLKL (S358) -
应用：
WB;IHC -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q8NB16 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to Phospho-MLKL(S358)
Description |
|---|
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process.Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. |
Specification |
|
|---|---|
| Aliases | hMLKL; Mlkl;;p-MLKL (S358) |
| Swissprot | Q8NB16 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human MLKL around the phosphorylation site of S358 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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