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Western blot analysis of PHF8 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Rabbit Monoclonal to PHF8
-
货号：
P17769 -
别名：
Histone lysine demethylase PHF8; MRXSSD; PHD finger protein 8; PHF8; ZNF422;;PHF8 -
应用：
WB,IF,IP -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9UPP1 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to PHF8
Description |
|---|
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. |
Specification |
|
|---|---|
| Aliases | Histone lysine demethylase PHF8; MRXSSD; PHD finger protein 8; PHF8; ZNF422;;PHF8 |
| Swissprot | Q9UPP1 |
| WB Predicted band size | Calculated MW: 118 kDa ; Observed MW: 140 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PHF8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
| IF | 1/50-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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