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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of PHD2 / prolyl hydroxylase expression in SH-SY5Y cell lysate.
Rabbit Monoclonal to PHD2/prolylhydroxylase
-
货号：
P17705 -
别名：
EGLN1; C1orf12; ECYT3; HIFPH2; HPH2; PHD2; SM-20; SM20; ZMYND6;;PHD2 -
应用：
WB;IP -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
Q9GZT9 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to PHD2/prolylhydroxylase
Description |
|---|
Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
Specification |
|
|---|---|
| Aliases | EGLN1; C1orf12; ECYT3; HIFPH2; HPH2; PHD2; SM-20; SM20; ZMYND6;;PHD2 |
| Swissprot | Q9GZT9 |
| WB Predicted band size | 46 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human PHD2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P17705
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