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Western blot analysis of Phospho-PKC alpha (T638) expression in (1) Jurkat cell lysate treated with TPA; (2) Jurkat cell lysate.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Rabbit Monoclonal to Phospho-PKC alpha(T638)
-
货号：
P17487 -
别名：
EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;;p-PKC alpha (T638) -
应用：
WB,IHC/IHF,IF,IP -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P17252 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to Phospho-PKC alpha(T638)
Description |
|---|
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. |
Specification |
|
|---|---|
| Aliases | EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;;p-PKC alpha (T638) |
| Swissprot | P17252 |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 80 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC alpha around the phosphorylation site of T638 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF | 1/50-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P17487
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