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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of PKC alpha expression in HEK293 cell lysate.
Immunofluorescent analysis of Hela cells, using PKC alpha Antibody .
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:100 dilution.
Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 100 dilution.
Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.
Rabbit Monoclonal to PKC alpha
-
货号：
P17143 -
别名：
EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;;PKC alpha -
应用：
WB,IHC/IHF,IF,IP,FCM -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P17252 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to PKC alpha
Description |
|---|
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. |
Specification |
|
|---|---|
| Aliases | EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-alpha; PRKACA; PRKCA; Protein kinase C; alpha type; kinase PKC-alpha;;PKC alpha |
| Swissprot | P17252 |
| WB Predicted band size | Calculated MW: 77 kDa ; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PKC alpha |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | 1/20-1/50 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF | 1/50-1/200 |
| FCM | 1/20-1/100 |
Product Image
- All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
- All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
- Western blot analysis of PKC alpha expression in HEK293 cell lysate.
- Immunofluorescent analysis of Hela cells, using PKC alpha Antibody .
- Immunofluorescent analysis using the Antibody at 1:50 dilution.
- Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:100 dilution.
- Immunohistochemical analysis of paraffin-embedded Human thymoma, using the Antibody at 1:200 dilution.
- Immunohistochemical analysis of paraffin-embedded Human melanoma, using the Antibody at 100 dilution.
- Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P17143
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