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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of NOTCH1 expression in HEK293 cell lysate.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis of HeLa cells, using Notch1 Antibody .
Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .
HOT
Rabbit Monoclonal to Notch 1
-
货号：
P17101 -
别名：
NOTC1, NOTCH1, TAN1, hN1, neurogenic locus notch homolog protein 1, neurogenic locus notch homolog protein 1 precursor,;Notch 1 -
应用：
WB,IHC/IHF,IF,FCM -
反应种属：
Human,Mouse -
抗体类型：
Primary antibody -
Swissprot：
P46531 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to Notch 1
Description |
|---|
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. |
Specification |
|
|---|---|
| Aliases | NOTC1, NOTCH1, TAN1, hN1, neurogenic locus notch homolog protein 1, neurogenic locus notch homolog protein 1 precursor,;Notch 1 |
| Swissprot | P46531 |
| WB Predicted band size | Calculated MW: 273 kDa ; Observed MW: 125 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Notch 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF | 1/50-1/200 |
| FCM | 1/20-1/100 |
Product Image
- All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
- Western blot analysis of NOTCH1 expression in HEK293 cell lysate.
- Immunofluorescent analysis using the Antibody at 1:50 dilution.
- Immunofluorescent analysis of HeLa cells, using Notch1 Antibody .
- Immunohistochemical analysis of paraffin-embedded human liver, using Notch1 Antibody .
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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