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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of RNF20 expression in (1) 293 cell lysate; (2) HeLa cell lysate.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
HOT

Rabbit Monoclonal to RNF20

  • 货号:

    P17013
  • 别名:

    BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20; ;RNF20
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IF,IP,FCM
  • 反应种属:

    Human,Mouse
  • 抗体类型:

    Primary antibody
  • Swissprot:

    Q5VTR2
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal to RNF20

Description

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation.

Specification

Aliases BRE1-A; hBRE1; RING finger protein 20; E3 ubiquitin-protein ligase BRE1A; RNF20; ;RNF20
Swissprot Q5VTR2
WB Predicted band size 114 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen A synthesized peptide derived from human RNF20
Formulation Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

Application

WB 1/1000-1/2000
IP 1/20-1/50
IF 1/50-1/200
FCM 1/20-1/100

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#P17013