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Microtiter wells were coated with human immunoglobulins with various isotypes. P16103 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Result: P16103 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.
Lane 1: 100 ng of human IgG1 ; Lane 2: 100 ng of human IgG2; Lane 3: 100 ng of human IgG3;Lane 4: 100 ng of human IgG4; Lane 5: 100 ng of human IgA ; Lane 6: 100 ng of human IgM ; Lane 7: 100 ng of human IgE ; Result: P16103 can specifically detect human IgG4 by Western blotting;
IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody (P16103)
IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue;Result: Squamous epithelial cells and non-germinal center cells are positively stained at cytoplasm.
Mouse Monoclonal Antibody to Human IgG4
-
货号：
P16103 -
别名：
-
应用：
WB,IHC -
反应种属：
Human -
抗体类型：
Primary antibody -
Swissprot：
P01861 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Mouse Monoclonal Antibody to Human IgG4
Specification |
|
|---|---|
| Swissprot | P01861 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Recombinant human IgG4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/500-1/1000 |
Product Image
- Microtiter wells were coated with human immunoglobulins with various isotypes. P16103 was used as primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Result: P16103 does not cross-react with human IgG1, IgG2, IgG3, IgA, IgM and IgE.
- Lane 1: 100 ng of human IgG1 ; Lane 2: 100 ng of human IgG2; Lane 3: 100 ng of human IgG3;Lane 4: 100 ng of human IgG4; Lane 5: 100 ng of human IgA ; Lane 6: 100 ng of human IgM ; Lane 7: 100 ng of human IgE ; Result: P16103 can specifically detect human IgG4 by Western blotting;
- IHC-P analysis of human tonsil tissue by anti-human IgG4 antibody (P16103) IHC-P was performed using sections of the formalin-fixed paraffin-embedded human tonsil tissue;Result: Squamous epithelial cells and non-germinal center cells are positively stained at cytoplasm.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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