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Rabbit Polyclonal Antibody to 6×His Tag
-
货号:
P00002 -
别名:
6 His epitope tag -
应用:
WB -
反应种属:
Fusion proteins containing the HIS Tag -
抗体类型:
Primary antibody -
Swissprot:
-
规格:
-
数量:
-+ -
说明书:
目录价¥1980

Rabbit Polyclonal Antibody to 6×His Tag
Description |
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The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
Specification |
|
---|---|
Aliases | 6 His epitope tag |
WB Predicted band size | 50/44 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Fusion proteins containing the HIS Tag |
Immunogen | Synthetic peptide: 18×His conjugated to KLH |
Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
Application |
|
---|---|
WB | 1/2000-1/10000 |
ELISA | 1/5000-1/10000 |
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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