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Western blot analysis of Phospho-p27 KIP 1 (Ser10) in Hela, 3T3 lysates using Phospho-p27 Kip 1 (Ser10) antibody.
Western blot analysis of Phospho-p27 KIP 1 (Ser10) in C6 lysates using Phospho-p27 KIP 1 (Ser10) antibody.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p27 KIP 1 (Phospho-Ser10) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Rabbit Monoclonal Antibody to Phospho-p27 Kip 1 (Ser10)

  • 货号:

    P20134
  • 别名:

    CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
  • 产品上架时间:

    2024-01-01
  • 应用:

    WB,IP,IHC
  • 反应种属:

    Human,Mouse,Rat
  • 抗体类型:

    Primary antibody
  • Swissprot:

    P46527
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Rabbit Monoclonal Antibody to Phospho-p27 Kip 1 (Ser10)

Description

The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Specification

Aliases CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Entrez GeneID 1027
Swissprot P46527
WB Predicted band size Calculated MW: 22 kDa; Observed MW: 27 kDa
Host/Isotype Rabbit IgG
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Rat
Immunogen A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Formulation Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol.

Application

WB 1/500-1/1000
IP 1/10-1/20
IHC 1/50-1/100