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Western blot analysis of lysates from MCF7 cells, using eIF2α Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody was used to detect the antibody.
Rabbit Monoclonal Antibody to eIF2α
-
货号：
50756 -
别名：
EIF2S1;EIF2A;Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha -
应用：
WB,IHC,IF,IP,ELISA -
反应种属：
Human,Mouse,Rat, -
抗体类型：
Primary antibody -
Swissprot：
P05198 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal Antibody to eIF2α
Description |
|---|
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010], |
Specification |
|
|---|---|
| Aliases | EIF2S1;EIF2A;Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha |
| Conjugation | Unmodified |
| Entrez GeneID | 1965 |
| Swissprot | P05198 |
| WB Predicted band size | 36kD |
| Host/Isotype | Rabbit IgG,Kappa |
| Antibody Type | Primary antibody |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Species Reactivity | Human,Mouse,Rat, |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
Application |
|
|---|---|
| WB | 1/2000-1/10000 |
| IP | 1/50-1/200 |
| IHC | 1/1000-1/5000 |
| IF/ICC | 1/200-1/1000 |
| ELISA | 1/5000-1/20000 |
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#50756
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