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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Western blot analysis of Histone H3 (acetyl K27) expression in HeLa cell treated with TSA cell lysate.
Rabbit Monoclonal to Histone H3(acetylK27)
-
货号：
P18735 -
别名：
Histone H3.1, Histone H3, HIST1H3A;;Acetyl-Histone H3 (K28) -
应用：
WB,IHC/IHF,IF,IP/ChIP -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P68431/P84243/Q16695/Q6NXT2/Q71DI3 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to Histone H3(acetylK27)
Description |
|---|
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Specification |
|
|---|---|
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;Acetyl-Histone H3 (K28) |
| Swissprot | P68431/P84243/Q16695/Q6NXT2/Q71DI3 |
| WB Predicted band size | Calculated MW: 15 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the acetylation site of K28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IP | ChIP:1/20-1/50 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF/ICC | 1/50-1/200 |
Product Image
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P18735
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