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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Western blot analysis of PUMA expression in K562 cell lysate.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Immunofluorescent analysis using the Antibody at 1:200 dilution.
Immunofluorescent analysis of Hela cells, using PUMA Antibody .
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PUMA Antibody.
Rabbit Monoclonal to PUMA
-
货号：
P17198 -
别名：
BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha;;BBC3 -
应用：
WB,IHC/IHF,IF,FCM -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
Q9BXH1 -
规格：
-
数量：
-+ -
说明书：
目录价¥2180
Rabbit Monoclonal to PUMA
Description |
|---|
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis.Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis. |
Specification |
|
|---|---|
| Aliases | BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha;;BBC3 |
| Swissprot | Q9BXH1 |
| WB Predicted band size | Calculated MW: 21 kDa ; Observed MW: 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BBC3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 |
| IF/ICC | 1/50-1/200 |
| FCM | 1/20-1/100 |
Product Image
- All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
- Western blot analysis of PUMA expression in K562 cell lysate.
- Immunofluorescent analysis using the Antibody at 1:50 dilution.
- Immunofluorescent analysis using the Antibody at 1:150 dilution.
- Immunofluorescent analysis using the Antibody at 1:200 dilution.
- Immunofluorescent analysis of Hela cells, using PUMA Antibody .
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using PUMA Antibody.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P17198
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