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Western blot analysis of GPR143 expression in THP1, RAW264.7 whole cell lysates.
Immunofluorescent analysis of GPR143 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue)
Rabbit Polyclonal Antibody to GPR143
-
货号：
P43647 -
别名：
OA1; G-protein coupled receptor 143; Ocular albinism type 1 protein -
应用：
WB,IF -
反应种属：
Human,Mouse,Rat -
抗体类型：
Primary antibody -
Swissprot：
P51810 -
规格：
-
数量：
-+ -
说明书：
目录价¥1980
Rabbit Polyclonal Antibody to GPR143
Specification |
|
|---|---|
| Aliases | OA1; G-protein coupled receptor 143; Ocular albinism type 1 protein |
| Entrez GeneID | 4935; |
| Swissprot | P51810 |
| WB Predicted band size | 43kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR143. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
Product Image
- Western blot analysis of GPR143 expression in THP1, RAW264.7 whole cell lysates.
- Immunofluorescent analysis of GPR143 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue)
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
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